Coding

Part:BBa_K5091009

Designed by: Angelina Oblak   Group: iGEM24_ULethbridge   (2024-09-30)


pmsB

The pmsB gene from Pseudomonas fluorescens A506 encodes an isochorismate pyruvate lyase (IPL), an enzyme involved in the biosynthesis of salicylic acid (SA). This gene, in conjunction with pmsC, is responsible for the conversion of chorismate to salicylic acid via a two-enzyme pathway, which is important for various biological processes, including plant defense mechanisms.

The first step in this pathway involves pmsC encoding an isochorismate synthase (ICS), which converts chorismate into isochorismate. pmsB then catalyzes the conversion of isochorismate into salicylic acid, which plays a significant role in plant-microbe interactions, especially in inducing systemic acquired resistance (SAR) in plants. The production of SA is crucial for activating plant immune responses, making this gene a valuable part for projects focused on enhancing plant defense against pathogens.

In practical applications, pmsB can be introduced into bacterial strains or other systems aiming to produce salicylic acid, either for boosting plant immunity or studying SA's role in biological processes. It is particularly useful in agricultural projects aimed at biocontrol or in synthetic biology applications where SA production is desired.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 91
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 91
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 91
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None